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Accelrys insightii software package
Insightii Software Package, supplied by Accelrys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/insightii+software+package/insight+ii+software/pm28019698-236-37-40
Average 90 stars, based on 1 article reviews
insightii software package - by Bioz Stars, 2026-10
90/100 stars

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Software:

Article Title: A new cytoplasmic monoheme cytochrome c from Acidithiobacillus ferrooxidans involved in sulfur oxidation.
Article Snippet: Acidithiobacillus ferrooxidans can obtain energy from the oxidation of various reduced inorganic sulfur compounds (RISCs, e.g., sulfur) and ferrous iron in bioleaching so has multiple branched respiratory pathways with a diverse range of electron transporters, especially cytochrome c proteins.. A cytochrome c family gene, afe1130, which has never been reported before, was found by screening the whole genome of A. ferrooxidans.. Here we report the differential gene transcription, bioinformatics analysis, and molecular modeling of the protein encoded by the afe1130 gene (AFE1130).

Article Title: Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase.
Article Snippet: .. Starting coordinates were extracted from the crystallographic structure of NpAS in complex with sucrose (PDB ID: 1JGI)46 to generate 3D models of the different enzymes of interest and mutations were introduced using the Biopolymer module of the InsightII software package (Accelrys). ..

Article Title: Computer-Aided Engineering of a Transglycosylase for the Glucosylation of an Unnatural Disaccharide of Relevance for Bacterial Antigen Synthesis
Article Snippet: The exploration of chemo-enzymatic routes to complex carbohydrates has been hampered by the lack of appropriate enzymatic tools having the substrate specificity for new reactions.. Here, we used a computer-aided design framework to guide the construction of a small, diversity-controlled library of amino acid sequences of an α-transglucosylase, the sugar binding subsites of which were re-engineered to enable the challenging 1,2-cis-glucosylation of a partially protected βlinked disaccharide allyl (2-deoxy-2-trichloroacetamido-β-D-glucopyranosyl)-(1→2)-α-L-rhamnopyranoside, a potential intermediate in the synthesis of Shigella flexneri cell-surface oligosaccharides.. The target disaccharide is not recognized by the parental wild-type enzyme and exhibits a molecular structure very distinct from that of the natural α(1→4)-linked acceptor.

Article Title: Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S -Transferase Enzyme Family
Article Snippet: .. Homology Modeling Three-dimensional structures of P. tabuliformis Tau GSTs were constructed using the InsightII software package (Accelrys) and x-ray structure of a soybean Tau GST (Protein Data Bank accession number 2VO4 ) as template. ..

Article Title: Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates
Article Snippet: .. Based on three-dimensional structures of AnGSTU3-6 and AnGSTU18-36 modeled using the InsightII software package (Accelrys, Inc., San Diego, CA), six amino acid sites were selected to create mutant proteins using site-directed mutagenesis for the biochemical assays. ..

Article Title: Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase
Article Snippet: Then, PreScission protease (Dutscher, Brumath, France) (500 U in 5 mL buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0)) was injected and cleavage of GST Tag occurred during 1 night always at 4°C. .. Starting coordinates were extracted from the crystallographic structure of Np AS in complex with sucrose (PDB ID: 1JGI) 46 to generate 3D models of the different enzymes of interest and mutations were introduced using the Biopolymer module of the InsightII software package (Accelrys). .. Protein concentration was estimated using Nanodrop ND‐1000 spectrophotometer.

Article Title: Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates.
Article Snippet: .. Based on three-dimensional structures of AnGSTU3-6 and AnGSTU18-36 modeled using the InsightII software package (Accelrys, Inc., San Diego, CA), six amino acid sites were selected to create mutant proteins using site-directed mutagenesis for the biochemical assays. ..

Mutagenesis:

Article Title: Computer-Aided Engineering of a Transglycosylase for the Glucosylation of an Unnatural Disaccharide of Relevance for Bacterial Antigen Synthesis
Article Snippet: The exploration of chemo-enzymatic routes to complex carbohydrates has been hampered by the lack of appropriate enzymatic tools having the substrate specificity for new reactions.. Here, we used a computer-aided design framework to guide the construction of a small, diversity-controlled library of amino acid sequences of an α-transglucosylase, the sugar binding subsites of which were re-engineered to enable the challenging 1,2-cis-glucosylation of a partially protected βlinked disaccharide allyl (2-deoxy-2-trichloroacetamido-β-D-glucopyranosyl)-(1→2)-α-L-rhamnopyranoside, a potential intermediate in the synthesis of Shigella flexneri cell-surface oligosaccharides.. The target disaccharide is not recognized by the parental wild-type enzyme and exhibits a molecular structure very distinct from that of the natural α(1→4)-linked acceptor.

Article Title: Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates
Article Snippet: .. Based on three-dimensional structures of AnGSTU3-6 and AnGSTU18-36 modeled using the InsightII software package (Accelrys, Inc., San Diego, CA), six amino acid sites were selected to create mutant proteins using site-directed mutagenesis for the biochemical assays. ..

Article Title: Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates.
Article Snippet: .. Based on three-dimensional structures of AnGSTU3-6 and AnGSTU18-36 modeled using the InsightII software package (Accelrys, Inc., San Diego, CA), six amino acid sites were selected to create mutant proteins using site-directed mutagenesis for the biochemical assays. ..

Construct:

Article Title: Computer-Aided Engineering of a Transglycosylase for the Glucosylation of an Unnatural Disaccharide of Relevance for Bacterial Antigen Synthesis
Article Snippet: The exploration of chemo-enzymatic routes to complex carbohydrates has been hampered by the lack of appropriate enzymatic tools having the substrate specificity for new reactions.. Here, we used a computer-aided design framework to guide the construction of a small, diversity-controlled library of amino acid sequences of an α-transglucosylase, the sugar binding subsites of which were re-engineered to enable the challenging 1,2-cis-glucosylation of a partially protected βlinked disaccharide allyl (2-deoxy-2-trichloroacetamido-β-D-glucopyranosyl)-(1→2)-α-L-rhamnopyranoside, a potential intermediate in the synthesis of Shigella flexneri cell-surface oligosaccharides.. The target disaccharide is not recognized by the parental wild-type enzyme and exhibits a molecular structure very distinct from that of the natural α(1→4)-linked acceptor.

Article Title: Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S -Transferase Enzyme Family
Article Snippet: .. Homology Modeling Three-dimensional structures of P. tabuliformis Tau GSTs were constructed using the InsightII software package (Accelrys) and x-ray structure of a soybean Tau GST (Protein Data Bank accession number 2VO4 ) as template. ..



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